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Orbigen
美國(guó)Orbigen www.orbigen.com
Orbigen位于風(fēng)景如畫的美國(guó)加州SAN DIEGO,其主要成員來自該地區(qū)著名的生物科技公司和大學(xué),在真核表達(dá),逆轉(zhuǎn)錄表達(dá),SiRNA,抗體開發(fā)等方面居于世界領(lǐng)先地位。著名品牌有:
Sapphire Baculovirus真核表達(dá)系統(tǒng)及預(yù)制基因庫(kù)
Orbigen公司可以提供幾百種重組的桿狀病毒表達(dá)載體,其涉及的外源基因主要來自于人、大鼠、小鼠、酵母、病毒等多種生物。除提供插入單個(gè)外源基因的重組桿狀病毒表達(dá)載體外,該公司還可以提供多種插入GST、His6標(biāo)記融合基因的重組桿狀病毒表達(dá)載體,極大地方便了客戶的科研工作。
Phoenix Retrovirus逆轉(zhuǎn)錄表達(dá)系統(tǒng)
該系統(tǒng)應(yīng)用非常廣泛,目前世界上有近2500家實(shí)驗(yàn)室采用該系統(tǒng)轉(zhuǎn)染將外源基因轉(zhuǎn)入分化的哺乳動(dòng)物細(xì)胞。采用LacZ和GFP基因作為報(bào)告系統(tǒng)。
試劑盒組分:
* PhoenixTM ampho 包裝細(xì)胞
* PhoenixTM eco 包裝細(xì)胞
* 10 μg pBMNZ 質(zhì)粒
* 10 μg pBMN-GFP 質(zhì)粒
BaculoBooster Virus Kit
該試劑盒包括10種不同的桿狀病毒庫(kù),每個(gè)桿狀病毒庫(kù)都含有表達(dá)一組真核生物伴侶蛋白的重組桿狀病毒混合物,這些伴侶蛋白可以在感染的昆蟲細(xì)胞(Sf9, Sf21 and T.ni)知道重組蛋白的折疊。該桿狀病毒庫(kù)可以與感染細(xì)胞內(nèi)的重組桿狀病毒相互作用,優(yōu)化目的蛋白的表達(dá)水平和折疊。
pCMV1/2/3 Xi-Clone 和pIX1/2/3 Xi-Clone 克隆表達(dá)系統(tǒng)
pCMV1/2/3 Xi-Clone?系統(tǒng)有3種不同的類型,每種類型分別包含一種pCMV載體,可以快速高效地實(shí)現(xiàn)體內(nèi)定向克隆。這類pCMV載體含有高度優(yōu)化的細(xì)胞巨化病毒(CMV)的啟動(dòng)子/增強(qiáng)子序列,因此表達(dá)效率比其它的CMV類載體(如:pcDNA3)要高好幾倍。
pIX1/2/3 Xi-Clone?含有有3種不同的類型(pIX、pIX2和pIX3),每種類型分別包含一種載體,可以快速構(gòu)建質(zhì)粒模板,實(shí)現(xiàn)體外的快速轉(zhuǎn)錄和翻譯。該類優(yōu)化的載體含有T7啟動(dòng)子,采用Xi-Clone定向克隆技術(shù)。
特點(diǎn):
* 3種不同的配置選擇:不標(biāo)記表達(dá)、N-端HA融合標(biāo)記和C-端HA融合標(biāo)記 。采用G418選擇穩(wěn)定轉(zhuǎn)染的細(xì)胞
* 利用anti-HA抗體可以快速檢測(cè)表達(dá)蛋白
Orbigen develops, manufactures and markets reagent tools for proteomic research. We are best known for novel antibodies, innovative gene expression technologies, and reliable custom protein expression services. By being at the forefront of technology development, we are proud to provide timely and quality products that help you continuously push the limits of what's possible.
Orbigen is a young and rapidly growing company headquartered in San Diego, California, one of the world's largest clusters of life science research institutes and companies. We employ an extensive group of dynamic and talented scientists who are empowered to create unique and exciting solutions to address your research needs. Many of our scientists are founding members and seasoned veterans from industry leaders. Our experienced technical staff strives to provide high-quality products and expert technical support to life science researchers worldwide.
In addition to providing unique research tools we can also help you commercialize your ideas and reagents. We have extensive experience and proven track record for working with inventors to timely and effectively bring important reagent to market.